Abstract: The external cables of Mars landing rover faces the extreme temperature, after “Tianwen-1” Mars landing rover lands on the surface of Mars. In allusion to cables would harden at low temperature, the materials of electric couplers and wires more easily damage when rotating at low temperature, the experiment method of low temperature adaptability of rotating cables is designed, to verify the adaptability and rotating performance of cables at Mars low temperature. The experimental results have testified that the cable assembly including electric couplers, wires and auxiliary materials are structurally integrated and electrically reliable after rotating at low temperature of Mars. Furthermore, the cables of spacecraft have experienced in-orbit flight, and have further evidenced the availability of this experiment method, and provide experiment method and technical support for the adaptability of cables to complex thermal environments of subsequent deep-space exploration.